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. 2021 Oct 7;8:698698. doi: 10.3389/fmolb.2021.698698

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Copyright © 2021 Hong, Zha, Wang, Qiao and An.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Verteporfin decreased the protective effect of folic acid on H9C2 cells by inhibiting YAP expression. (A) The effect of verteporfin on the percentage of viable H9C2 cells. (B) Representative immunoblot showing expression levels of YAP and p-YAP in H9C2 cells treated with verteporfin. (D, G) Representative immunoblot showing levels of NLRP3, ACS, Cleaved-Caspase-1, Caspase-1, YAP and p-YAP. (H) IL-1β and IL-18 levels in the cell supernatant. (C, E, F) Quantification of YAP1, p-YAP1, NLRP3, ACS, with α-tubulin as the loading control, for Cleaved-caspase-1, Caspase-1 as the loading control. ^* p < 0.05, compared with NG; ^# p < 0.05, compared with HGF, ^& p < 0.05, compared with HGF + Folic acid.