Subject	Biomaterials
Specific subject area	Biomaterials applied in tissue engineering
Type of data	Tables Images
How data were acquired	CS and CS-GO molecular weight were measured by Gel Permeation Chromatography (GPC) according to the USP guide, using two columns Shodex Ohpak 805 and 806, with many theoretical plates < 10,000. The measurements were carried out in an Agilent Infinity 1260 (Agilent Technologies, Santa Clara, California, USA) with a refractive index detector. For the biocompatibility assay, brine shrimp eggs were culture into a box with culture wells, a membrane of CS-GO, and the physical mix of CS and GO (CS/GO) were used in some wells, and living larvae were counted at 24, 36 and 48 h. For the growth cell test, a Leica inverted field microscope (Leica Microsystems, Mannheim, Germany) took images and a Neubauer camera (Blood Counting Chambers, BOECO, Hamburg Germany) to count cells.
Data format	Raw Analyzed
Parameters for data collection	For molecular weight measurements, it was necessary to dissolve samples into the mobile phase in a 1 mg/mL concentration and then filter it with a PVDF membrane of 0.45 µm. The parameters to which GPC analysis was carried out were a rate of 0.5 mL/min, a column temperature of 35 °C, and a sample volume of 20 µL. For the biocompatibility test, the Rocha-Filho protocol [1] was followed. For this, brine shrimp eggs were hydrated with seawater and cultured in a box with wells for cell culture. For the test, it was necessary membranes of CS-GO and CS/GO, one membrane was located in the well, and the biocompatibility was determined by counting living larvae. For the growth cell test, unfreeze cells were taken to a New Brunswick Galaxy 48R incubator with a complete culture medium. Eight membranes of CS-GO were deposited in a box with 12 culture wells with approximately 30,000 cells. After nine days, cells were detached, followed images were taken using a Leica inverted field microscope to verify cell growth. Finally, the number of cells was counted using a Neubauer camera.
Description of data collection	Cell images were taken using a Leica inverted field microscope, and a Neubauer camera obtained the number of cells.
Data source location	Universidad del Valle Cali, Valle del Cauca, Colombia Gingival fibroblasts were obtained from the cell bank of the in-vitro Cell Culture Laboratory of the Universidad del Valle in Cali, Colombia.
Data accessibility	With the article
Related research article	Valencia, A. M.; Valencia, C. H.; Zuluaga, F.; Grande-Tovar, C. D. Synthesis and Fabrication of Films Including Graphene Oxide Functionalized with Chitosan for Regenerative Medicine Applications. Heliyon 2021, 7 (5), e07058. 10.1016/j.heliyon.2021.e07058.