Figure 3.

Excessive cellular influx of inflammatory monocytes and neutrophils in Tpl2^-/- mice infected with influenza. WT and Tpl2^-/- mice were infected intranasally with 10⁴ pfu of influenza x31 and euthanized at 7 dpi. The lungs were lavaged, perfused with PBS, digested with collagenase, and interstitial leukocytes were enriched by Percoll density gradient centrifugation (A–H). Cell populations of infected WT (n = 6) and Tpl2^-/- (n = 5) mouse lungs (post lavage, perfusion and digest) at 7 dpi are shown. Data are representative of 3 experiments. (I, J) Infiltrating cell populations were also assessed for WT (n = 7) and Tpl2^-/- (n = 8) mice at 4 dpi (post lavage, perfusion and digest), including uninfected controls (UI). Data are representative of 3 experiments. Unpaired student’s t-test *p<0.05, **p<0.01, ***p<0.001. Squares represent male mice, and circles represent female mice.