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. 2021 Oct 7;12:738490. doi: 10.3389/fimmu.2021.738490

Figure 4.

Figure 4

Increased mRNA expression of proinflammatory mediators in lungs of influenza-infected Tpl2-/- mice at 7 dpi. (A–R) WT (n = 10) and Tpl2-/- (n = 9) mice were infected intranasally with 104 pfu of influenza x31 and euthanized at 7 dpi. The lungs were homogenized, and RNA was extracted and analyzed for gene expression by real-time PCR for pro-inflammatory cytokines (A–F), chemokines (G–K), anti-inflammatory cytokine (L), inflammatory mediators (M–P) and transcriptional regulators (Q, R). Unpaired student’s t-test; *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001). (S) Pearson’s correlation of CCL2 mRNA versus IFN-β mRNA on day 7. *p<0.05 (T) Pearson’s correlation of NOS2 mRNA versus CCL2 mRNA on day 7. *p<0.05 (U) Pearson’s correlation of NOS2 mRNA versus IFN-β mRNA on day 7. *p<0.05 (V) Pearson’s correlation of CXCL1 mRNA versus IFNβ mRNA on day 7. *p<0.05 (W) Pearson’s correlation of NOS2 mRNA versus CXCL1 mRNA on day 7. *p<0.05 (X) Pearson’s correlation of NOS2 mRNA versus IFN-γ mRNA on day 7. *p<0.05 Data are representative of 2 experiments. Squares represent male mice, and circles represent female mice.