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. 2021 Aug 30;35(10):e23986. doi: 10.1002/jcla.23986

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© 2021 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Anlotinib and gemcitabine inhibit cell growth of ICC cell lines in vitro. (A and B) HCCC‐9810 and RBE cells were treated with anlotinib or gemcitabine at the indicated concentrations for 24, 48, and 72 h. The CCK‐8 assay measured the growth inhibition rate. (C and D) HCCC‐9810 and RBE cell lines were treated with the combination of anlotinib and gemcitabine for 48 h by constant dilution, and then the growth inhibition rate of ICC cell lines was detected by CCK‐8. The CI of the two drugs was calculated by Compusyn software, and the value of CI less than 1.0 indicated a synergistic effect (n = 3). Con: control; An: anlotinib; Gem: gemcitabine