Figure 4.

No evidence of time-course signal discrimination. (A) The same experimentally measured data from mouse B cells presented in Figure 2D is re-plotted here as time courses for IFNα2 and IFNβ at comparably low (~10¹ pM) and high (~10³ pM) doses. There is no apparent difference in the character (transient versus sustained) responses between IFN subtypes in either concentration regime. (B) The measured pSTAT response at early (5 minutes) and late (60 minutes) times, at equal concentrations, are plotted with the early-time response on the horizontal axis and late-time response on the vertical axis. A sustained response would produce points on the diagonal (black dashed line) while a transient response would produce points away from the diagonal. The points are not separable as transient versus sustained response based on the type of IFN, within the error bars.