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. 2021 Oct 7;11:710525. doi: 10.3389/fonc.2021.710525

Figure 1.

Figure 1

ZNF304 downregulation in ccRCC is correlated with good prognosis. (A) Staining of Hematoxylin and eosin in ccRCC (T) and normal (N) kidney tissues. Scale Bar = 50 μm. (B) RT-qPCR was use to examine ZNF304 mRNA level in ccRCC (T, n = 43) and normal kidney (N, n = 43) tissues. (C) Western blot analysis detected the protein levels of ZNF304 in ccRCC (T) and normal (N) kidney tissues (D) Quantitative analysis of (C). (E) ZNF304 mRNA levels were analyzed from data of the TCGA database. (F) Kaplan–Meier analysis of the overall survival of ccRCC patients in the TCGA database with low (n = 130) and high (n = 130) ZNF304 levels (cutoff value is 25%). (G) Western blot was used to measure the proteins level of ZNF304 in 293A and RCC cell lines (A498, 786-0, and SW839). (H) Quantitative analysis of (G). (I) qRT-PCR was performed to detect the ZNF304 mRNA level in the above cell lines. All data are from three independent experiments and expressed as the mean ± SEM. *P < 0.05, **P < 0.01 vs. the corresponding controls.