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. 2021 Oct 7;11:710525. doi: 10.3389/fonc.2021.710525

Figure 3.

Figure 3

FOXO4 regulates ZNF304-mediated cell proliferation. (A) 786-0 and SW839 cells were transfected with pKLO, shZNF304, pWPI, and oeZNF304 vectors, and then protein expression of FOXO4 was measured by western blot. (B) Quantitative analysis of (A). (C) The expression of FOXO4 mRNA was measured by qRT-PCR in ccRCC (T, n = 43) and normal kidney (N, n = 43) tissues. (D) the overall survival of ccRCC patients from the TCGA database was analyzed by Kaplan–Meier analysis. FOXO4 levels low (n = 130) and high (n = 130) (cutoff value is 25%). (E) The correlation between ZNF304 and FOXO4 mRNA expression in PCa tissues was analyzed by Pearson correlation analysis of our clinical data (R = 0.4073, P < 0.05). (F) 786-0 and SW839 cells were transfected with oeZNF304, shFOXO4, oeZNF304 + shFOXO4 and control vectors, and cell viability was measured by MTT. (G) Cells were prepared as in (A), and FOXO4 mRNA was detected by RT-qPCR. (H) Cells were prepared as in (A), and cell viability was measured by MTT (D) and colony formation assays (E, F). All data are from three independent experiments and expressed as the mean ± SEM. *P < 0.05, **P < 0.01 vs. the corresponding controls.