Skip to main content
. 2021 Oct 7;11:710525. doi: 10.3389/fonc.2021.710525

Figure 5.

Figure 5

ZNF304 downregulates miR-183-5p expression by transcriptional repression. (A) The level of miR-183-5p was detected by qRT-PCR in ccRCC (T, n = 43) and normal kidney (N, n = 43) tissues. (B) According to the expression level of miR-183-5p in the TCGA database, kaplan-Meier was used to analyze the overall survival of ccRCC patients (cutoff value is 25%). (C) Pearson correlation analysis ZNF304 and miR-183-5p correlation (R = 0.4299, P = 0.0158). (D) JASPAR (http://jaspar.genereg.net/) predicted the potential binding site for ZNF304 in the miR-183-5p promotor. (E) Binding site of ZNF304 in miR-183-5p promoter region was confirmed by using ChIP-qPCR in 786-0 cell. The position of the primer is indicated by the arrowheads. (F) 786-0 and SW839 cells were transfected with oeZNF304 or shZNF304 or control vectors, and pre-miR-183-5p expression was measured by RT-qPCR. All data are from three independent experiments and expressed as the mean ± SEM. *P < 0.05, **P < 0.01 vs. the corresponding controls.