Figure 6.

ZNF304/miR-183-5p/FOXO4 axis inhibits ccRCC progression. (A, B) 786-0 cells were transfected with the oeZNF304, 183-5p inhibitor or co-transfected with both and then subjected to western blot analysis the FOXO4 and Cyclin D1 protein level. (C, D) Indicated RNA and vectors were used to transfect into 786-0 cells and the FOXO4 and Cyclin D1protein level were detected by Western blot. (E–G) xenograft tumors were performed by injecting 786-0 cells with stably downregulated miR-183-5p in nude mice and tumor volumes (E, F) or tumor weight (G) were measured by direct measurement. (H) FOXO4 and Cyclin D1 proteins level in xenograft tumors were examined by using western blot. All data are from three independent experiments and expressed as the mean ± SEM. *P < 0.05, **P < 0.01 vs. the corresponding controls.