FIG. 2.

Characterization of an antibody raised against the C terminus of MBD1. (A) Anti-MBD1 antibody detects two major forms of MBD1 in HeLa nuclear extract (thick arrows; right panel). Adsorption of the antibody with the cognate antigen (GST-MBD1) drastically reduces the reaction of the serum with recombinant MBD1 and prevents the detection of MBD1 in a HeLa nuclear extract (left panel). Mock adsorption with GST does not affect the reactivity of the serum toward MBD1. The asterisk denotes a nonspecific band due to secondary antibody. HeLa NE denotes 30 μg of HeLa nuclear extract. (B) Anti-MBD1 antibody recognizes MBD1 but not MBD2b, MBD4, or bovine serum albumin.