FIGURE 4.

No significant age-related differences were seen in the percentage of CD45-positive cells in colon sections in ascending or descending colon. Panels (A–C) show methods used to calculate the percentage of CD45-positive cells. A bin for analysis was taken (A), and thresholding was used to identify CD45-positive cells and nuclei, which were then counted. Statistical tests compared results for each colon region between age groups; adult (25–60 years old; •) and elderly (70 + years old; ▲). Bars indicate data mean. Data was collected separately for: longitudinal muscle, circular muscle near the myenteric plexus, and circular muscle near the mucosa. Data shown represents an average from the three regions from each individual. No statistical significance was found when comparing age groups for the three regions individually, or when grouped. Data was analyzed by two independent observers. Results from one observer are shown in panel (D) as an example.