Summary of % bacterial phospholipid membrane compositions. Where ‘—’ is shown, the presence of this phospholipid was not confirmed or was shown to be absent within experimental limitations. Where the phospholipid composition does not equal 100%, the remaining phospholipids either could not be identified, or the membrane is also comprised of some less prevalent phospholipids which have been omitted from this summary table. These less common phospholipids include: PA, PC, PS, L-PE, L-CL. For full details, including those relating to alkyl chain composition, culture conditions and phospholipid source, please see Table S1a.
| Bacterial strain | PG | L-PG | DAG | CL | PE | Growth phase | Analysis method |
|---|---|---|---|---|---|---|---|
| E. faecium (Gram +ve) | |||||||
| R446 (ref. 66) | 15 | 16 | 23 | 47 | — | Stationary | MS |
| S447 (ref. 66) | 34 | 14 | 13 | 29 | — | Stationary | MS |
| S. aureus (Gram +ve) | |||||||
| USA300 (ref. 67) | 60 | 30 | 4 | 2 | — | Log | MS |
| USA300 (ref. 67) | 62 | 20 | 3 | 4 | — | Stationary | MS |
| DSM 20233 (ref. 68) | 50 | 20 | — | 1 | — | Log | TLC |
| fakA mutant67 | 70 | 25 | 3 | 1 | — | Log | MS |
| fakA mutant67 | 60 | 22 | 5 | 6 | — | Stationary | MS |
| CB1118 (ref. 69) | 84 | 12 | — | 7 | — | Unknown | TLC |
| CB2205 (ref. 69) | 84 | 25 | — | 7 | — | Unknown | TLC |
| 209P70 | 79 | 14 | — | 4 | — | Log | TLC |
| 209P70 | 66 | 10 | — | 5 | — | Stationary | TLC |
| U-71 (ref. 58) | 80 | 12 | — | 5 | — | Unknown | TLC |
| K. pneumonia (Gram −ve) | |||||||
| Smooth mutant71 | 5 | — | — | 6.5 | 82 | Unknown | TLC |
| 005 (ref. 72) | 35 | — | — | — | 59 | Unknown | MS |
| A. baumannii (Gram −ve) | |||||||
| HO1–N73 | 10 | — | — | 7 | 73 | Unknown | GC |
| P. aeruginosa (Gram −ve) | |||||||
| PAO1 (ref. 74) | 27 | — | — | — | 69 | Stationary | MS |
| B-219 (ref. 75) | 41 | — | — | — | 65 | Unknown | MS |
| Enterobacter cloacae (E. cloacae) (Gram −ve) | |||||||
| Sw1 (ref. 76) | 23 | — | — | 3.5 | 73 | Stationary | TLC |
| Sw1 (ref. 76) | 6 | — | — | 8 | 77 | Log | TLC |
| 012 (ref. 72) | 35 | — | — | — | 65 | Unknown | MS |
| 008 (ref. 72) | 42 | — | — | — | 50 | Unknown | MS |
| AZT-R76 | 17 | — | — | 2 | 80 | Log | TLC |
| AZT-R76 | 5 | — | — | 9 | 76 | Stationary | TLC |
| AMA-R76 | 17 | — | — | 3 | 79 | Log | TLC |
| AMA-R76 | 5 | — | — | 6 | 82 | Stationary | TLC |
| B. subtilis (Gram +ve) | |||||||
| I′1a (ref. 77) | 70 | 3 | — | 4 | 22 | Log | MS |
| I′1a (ref. 77) | 75 | 2 | — | 7 | 17 | Stationary | MS |
| DSM 3257 (ref. 77) | 65 | 2 | — | 4 | 35 | Log | MS |
| DSM 3257 (ref. 77) | 30 | 2 | — | 10 | 70 | Stationary | MS |
| E. coli (Gram −ve) | |||||||
| B78 | 20 | — | — | 5 | 75 | Log | TLC |
a
MS = mass spectrometry. TLC = thin layer chromatography, GC = gas chromatography.