Summary of cancer cell phospholipid membrane composition. Upwards arrow indicates an upregulation of specific phospholipids, downwards arrow indicates a downregulation of specific phospholipids. Where there are both upwards and downwards arrows present, this reflects the fact there will be multiple forms of a particular phospholipid present in a cell, due to different alkyl chain lengths and their degree of saturation. Some of these maybe upregulated in a cancer cell, whereas others maybe downregulated. Where ‘—’ is shown, a change in phospholipid content was either not observed or not confirmed within experimental limitations. Tissue refers to human tissue obtained from clinical samples, unless otherwise stated. The cell membranes listed here may also contain some less prevalent phospholipids. These data, alongside alkyl chain composition and, the results of studies that restrict analysis to a limited number of phospholipids, are listed in Table S2. These less common phospholipids include: L-PA, L-PC, and L-PI, L-SM. Phospholipid composition was estimated through mass spectrometry (MS).

Sample type	PC	PE	PI	PG	PS	SM	PA	Control
Lung
Tissue92				—			—	Matched distant lung tissue
Tissue93			—	—	—	—	—	Distal non-cancerous tissue
Tissue94				—	—			Adjacent normal tissue
Tissue95		—	—	—	—		—	Adjacent normal lung tissue
Tissue96		—	—	—	—		—	Normal lung tissue
Breast
Cell lines97				—	—		—	MCF10A cell line
Cell lines97				—	—		—	MCF10A cell line
Cell lines97		—		—	—		—	MCF10A cell line
Cell lines98						—	—	MCF10A cell line
Cell lines98						—	—	MCF10A cell line
Tissue94				—	—			Adjacent normal tissue
Tissue99				—	—		—	Adjacent normal tissue
Tissue100		—	—	—	—		—	Adjacent normal breast tissue
Tissue101				—	—		—	Normal breast tissue
Prostate
Cell lines102			—	—	—		—	PNT1a cell line
Tissue103				—	—		—	Tissue from healthy males
Colorectal
Tissue104	—	—	—			—		Adjacent normal mucosa
Tissue94				—	—			Adjacent normal tissue
Tissue105				—		—	—	Adjacent normal mucosa
Tissue106			—	—	—	—	—	Adjacent normal mucosa
Tissue107			—				—	Tumour adjacent tissue
Colorectal liver metastasis
Tissue108				—			—	Normal liver parenchyma
Ovarian
Tissue109		—		—	—	—	—	Adjacent normal tissue
Urothelial cancer of the bladder
Tissue110				—		—	—	Benign adjacent tissue
Gastric
Tissue94				—	—			Adjacent normal tissue
Oesophageal
Tissue94				—	—			Adjacent normal tissue
Tissue111	—					—		Matched healthy oesophageal epithelium
Thyroid
Tissue94				—	—			Adjacent normal tissue
Hepatocellular
Tissue112			—	—	—		—	Benign tissue