Table 1. The information of primer sequences for selected SNPs.
Primer name | Primer sequence | Amplified length | Restriction enzyme |
---|---|---|---|
rs4842194-F | 5′-CCGTCCTGAGGCAGAGTGTTG-3′ | 299 bp | Pst I |
rs4842194-R | 5′-TCCCAAGAAGCAGCCGACC-3′ | ||
rs100537-F | 5′-CCTGGCACGGTGACTTGA-3′ | 667 bp | Msp I |
rs100537-R | 5′-TGGGAAATACGAATGACTGGAT-3′ | ||
rs2134095-F | 5′-CTTGCTGTGCCTGTTG-3′ | 525 bp | Hinf I |
rs2134095-R | 5′-GTGGAAGAGGGCTGAA-3′ |
Abbreviations: F, the forward primer; R, the reverse primer.