Fig. 6.

Eradication of biofilm by PDMS-PIM1. (A) Biofilms were formed on glass cover slips. PDMS and PDMS-PIM1 material were overlaid on the biofilm and incubated at 35 °C for respective time points. The biofilm bacteria inoculate was harvested in 10 ml PBS and plated on LB agar plates following appropriate dilution. (B) PDMS material and PDMS-PIM1 material were immersed in 10 ml PBS. Tubes (2 cm × 3) with preformed biofilm in lumen were then incubated in the presence of PDMS-PIM material of varying weights, for 1 h at 100 rpm/35 °C. The viable colonies were determined by plate assay in both experiments. The results are expressed as mean and standard deviation of three replicates. * denotes zero (0) CFU on the agar plate for undiluted samples