Figure 1.
FPC in Pkhd1del4/del4(del4) cholangiocytes. (A) Western blotting for FPC. Please note that del4 cells have substantially less FPC. Antibody was obtained from the Baltimore PKD Center. α-Tubulin was used as the loading control. (B) Summary. Reduced CFTR in del4 cholangiocytes. (C) Western blot showing CFTR. (D) Summary. (E, F) Sox9: WT, del4, and VX-809–treated del4 cholangiocytes stained for Sox9 (red), expressed as mean intensity. (G) Western blotting for Sox9. (H) Summary data. Number of Western blot experiments represented in the figure (dots) are from independent 10-cm dishes of cultured cells. Student’s t test. ∗P < .05; ∗∗∗P < .001; ∗∗∗∗P < .0001.