Figure 4.

ERAD protein expression in ARPKD cells. (A) Western blot of HSP27, HSP70, and HSP90 in WT or del4 cholangiocytes and del4 cells treated with VX-809 (10 μM). α-tubulin was used as the loading control. (B–D) Summary data. All data are expressed as mean ± SEM. P values were determined by Student’s t test. Please note that the same gel for HSP70 is presented at 2 different exposure times. Number of Western blot experiments represented in the figure (dots) are from independent 10-cm dishes of cultured cells. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001.