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. 2021 Oct 2;24(11):103211. doi: 10.1016/j.isci.2021.103211

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© 2021 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Single-particle analysis of intact ribosomal particles by MP and Orbitrap-based CDMS

(A) Cartoons depicting the structures of the Hs40S (left; PDB: 5A2Q), Hs80S (center; PDB: 4UJC), and So70S (right; PDB: 5MMM) ribosomes.

(B) Mass distribution histograms obtained for the Hs40S (left), HS80S (center), and So70S (right) ribosomal particles using MP (top) and CDMS (bottom). The fitted mean mass and standard deviation of the most abundant species are provided, using the same color coding for MP and CDMS data. Normalized counts (%) for each detected species in the displayed mass ranges are indicated within each fitted normal distribution. See also Figure S2 showing single-molecule mass analysis of Hs60S and Table S5 containing technical details for each measurement visualized in (B).

(C) 2D histogram representations of the CDMS data with color-coded and annotated co-occurring particle detection events, showing the improved separation of distinct ribosomes achieved by using both the z and m/z dimensions.