Figure 4.

Metrics of performance in CDMS and MP

(A) Experimental peak width (expressed as FWHM) for all measured ribosomal particles with CDMS (red dots) and MP (purple dots) reveals that CDMS achieves substantially higher resolution, especially for the larger particles analyzed (inset). See also Table S5.

(B) Mass accuracy determined by CDMS and MP for Hs40S, Hs80S, and So70S ribosomal particles. The dash-dot-dash, dashed, and solid lines provide the 1% mass threshold for the Hs40S, So70S, and Hs80S particles, respectively.

(C) The contrast-to-mass calibration curves produced for mass determination with MP either using the manufacturer-recommended native marker protein standard (dashed gray line) alone or extended with either in-house analyzed MDa proteinaceous particles (I53-40 nanoparticles and adeno-associated virus AAV8 particles, solid black line) or human ribosomal particles (Hs40S, Hs60S, and Hs80S, solid blue line).