Table 1.
Overview of the ribosomal samples analyzed by MP and CDMS
| Hs40S | Hs60S | Hs80S | So30S | So50S | So70S | |
|---|---|---|---|---|---|---|
| Organism | H. sapiens | H. sapiens | H. sapiens | S. oleracea | S. oleracea | S. oleracea |
| Subcellular location | Cytosol | Cytosol | Cytosol | Chloroplast | Chloroplast | Chloroplast |
| Number of proteins | 34 | 47 | 81 | 25 | 34 | 59 |
| rRNA fragments | 18S (HCV IRES RNA) | 28S, 5S, 5.8S | 18S, 28S, 5S, 5.8S | 16S | 23S, 4.5S, 5S | 16S, 23S, 4.5S, 5S |
| Theoretical mass (kDa) | 1,214 (1,317)b | 2,633 | 3,847 | 909 | 1,539 | 2,448 |
| Mass from Native MS (kDa) | 1,220 (1,324)b | 2,585 | –a | 861 | 1,456 | 2,353 |
See also Figures S1 and S3, and Tables S1–S4.
a
Could not be measured by standard native MS, but a mass of 3,805 kDa is suggested by summing up the Hs40S and Hs60S masses.
b
The mass in parenthesis is that of the IRES-bound Hs40S ribosomal particles.