Table 2.

An overview of strengths and weaknesses for different mass analytical approaches

	MP	Orbitrap-based CDMS	Conventional native MS
Environment	Solution phase	Gas phase	Gas phase
“Nativeness”	+++	+	+
Typical acquisition time	∼5 min	∼10–60 min	∼5–15 min
Processing software	Commercial	Custom scripts	Commercial
Sample consumption	1–10 nM	10 nM–1 μM	1–10 μM
Sample volume	1–10 μL	1–10 μL	1–10 μL
Resolution	∼4	∼10	∼100–200a
Accuracy	<2%	<2%	<1%b
Typical molecule count	1e3-4	1e4-5c	>1e6
Max reported mass	∼5 MDad	∼10 MDa	∼10 MDa
Detection limitations	<50 kDa, low Kd	z < 10+, buffer	High heterogeneity, buffer
Dynamic range	+	++	+++
Cost	$	$$$	$$$

^aBased on the data from Figures S1 and S3.

^bRequires charge-state separation and is based on Van De Waterbeemd et al. (2017).

^cAfter data filtering.

^dReported by the vendor.