Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Oct 20;13:196. doi: 10.1186/s13148-021-01177-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 6 — MeD-seq results for published biomarkers on cfDNA from HBDs and paired tissue, pre-operative and post-operative cfDNA from CRLM patients. A Sequenced MeD-seq reads for EYA4, GRIA4, MSC, and ITGA4 of CRLM tissue (white), pre-operative cfDNA (red), and post-operative cfDNA (orange) from CRLM-patients and HBDs (black). Results are visualized in Integrative Genomics Viewer (IGV) v.2.9.4. The green bars indicate the locations of the respective qMSP amplicons. B qMSP-based methylation levels relative to the ACTB reference gene are shown for EYA4 (top-left), GRIA4 (top-right), MSC (bottom-left), and ITGA4 (bottom-right) at baseline (BL) and 5 days post-surgery (+ 5d) in cfDNA of 10 CRLM patients and 3 healthy blood donors (HBDs). A median decrease in methylation levels was observed for EYA4, GRIA4, MSC, and ITGA4 5 days after surgery compared to BL in CRLM patients (exact sign test; p = 0.004, p = 0.07, p = 0.002, and p = 0.039, respectively)