Table 4.

List of strains used in the present study. All listed strains are “mono-eukaryotic clonal cultures”, type of in vitro cultivation, xenic or monoxenic, is indicated. All H. meleagridis cultures were established in our laboratory from caecal material and faeces of infected turkeys or chickens that were sent to our Clinic (Clinic for Poultry and Fish Medicine, University of Veterinary Medicine, Vienna, Austria) for routine diagnostic investigation. The host bird species for each culture is indicated in the assignment of the strain according to following scheme: Histomonas meleagridis /bird species from which he parasite was isolated/country/diagnostic number – clone no. at micromanipulation/year of isolation

strain	type of in vitro cultivation	passage	purpose
H. meleagridis/turkey/Austria/2922-C6/04/DH5αa	monoxenic	28p	Illumina and MINIon sequencing, confirmation of variants
		346p
H. meleagridis/turkey/Austria/2922-C6/04b	xenic	13p	confirmation of variants
		51p
		83p
		145p
		237p
		292p
H. meleagridis/chicken/Austria13250-C20/10c	xenic	24p
		316p
H. meleagridis/turkey/Austria/2877-C3/05c	xenic	22p
H. meleagridis/chicken/Austria/8175-C7/06c	xenic	21p

^aassignment: Histomonas meleagridis isolated from turkey/country/diagnostic number – clone no. at micromanipulation/year of isolation/monoxenic grown with E. coli DH5α. Monoxenization was done independently at passage 10 (virulent) and 290 (attenuated) [13]

^bxenic cultures prior to monoxenization at passage 290

^c assignment Histomonas meleagridis /bird species from which he parasite was isolated/country/diagnostic number – clone no. at micromanipulation/year of isolation