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. Author manuscript; available in PMC: 2021 Oct 21.
Published in final edited form as: Sci Immunol. 2016 Nov 18;1(5):eaaf8471. doi: 10.1126/sciimmunol.aaf8471

Fig. 1. TSLP increases MRSA killing in mouse blood in a neutrophil-dependent manner.

Fig. 1

(A, C, D) Mouse blood was incubated with PBS or TSLP and MRSA for 3 h. (A) CFU analysis (shown is a representative experiment from blood from 2 mice performed in triplicate). (B) TSLPR expression on mouse neutrophils incubated with medium or HKSA. (C) Flow-cytometric staining of blood neutrophils in mice treated with a control antibody or depleted of neutrophils using anti-Ly6G antibody. (D) A representative experiment showing CFU of MRSA after an in vitro whole blood killing assay performed with blood from mice treated with an isotype control or anti-Ly6G antibodies (blood from 2–3 mice were combined for each treatment condition and assayed in triplicate). *, p < 0.05; ***, p < 0.001; ns = not significant, two-tailed Student’s t-test. Data are representative of 6 independent experiments for (A), 2 experiments for (B) and 3 experiments for (C) and (D).