(A, C, D) Mouse blood was incubated with PBS or TSLP and MRSA for 3
h. (A) CFU analysis (shown is a representative experiment from
blood from 2 mice performed in triplicate). (B) TSLPR expression on
mouse neutrophils incubated with medium or HKSA. (C)
Flow-cytometric staining of blood neutrophils in mice treated with a control
antibody or depleted of neutrophils using anti-Ly6G antibody. (D) A
representative experiment showing CFU of MRSA after an in vitro
whole blood killing assay performed with blood from mice treated with an isotype
control or anti-Ly6G antibodies (blood from 2–3 mice were combined for
each treatment condition and assayed in triplicate). *, p <
0.05; ***, p < 0.001; ns = not
significant, two-tailed Student’s t-test. Data are
representative of 6 independent experiments for (A), 2 experiments for (B) and 3
experiments for (C) and (D).