(A–G) Mice were infected with MRSA i.d. in the ear.
(A) TSLP protein expression in the ear after i.d. MRSA
infection (n=4), naïve controls were
mock-infected with PBS only. (B–G) Ears were analyzed on
day 1 p.i. (B) Representative TSLPR expression on neutrophils.
(C) Analysis of CFU in the ear of WT
(n=11) and
Tslpr−/− mice
(n=12 ears). (D–F) Analysis of
neutrophils in the ear. Shown are representative FACS plots (D) and
percent (E) and total number (F) of neutrophils from
WT and Tslpr−/− mice
(n=8 ears). (G) CFU of MRSA in the ear
on day 2 post-i.d. ear infection of WT mice treated with human IgG1 Fc isotype
control or TSLPR Fc (two-tailed Mann-Whitney test) (n=8
ears). *, p < .05; **, p < .01;
***, p < .001; ns = not significant.
a–f, two-tailed Student’s
t-test. Data are representative of 3 (or 2 for
(G)) independent experiments.