(A–C) Day 1 p.i. of mice infected with MRSA i.d. in the ear.
(A–B) ROS production of mouse neutrophils after
staining with CellROX deep red. Shown are a representative FACS plot
(A) and mean fluorescence intensity (MFI) (B) of
WT (n=6) and
Tslpr−/− mice
(n=8 ears). (C) Mice were injected
i.d. in the ear with MRSA and either PBS or TSLP along with either control (PBS)
or a ROS inhibitor (NAC). CFU on day 1 p.i. (two-tailed Mann-Whitney test,
n=16 ears). (D) CFU in the ear on day
2 p.i. of WT and Gp91 phox−/− mice
infected with MRSA and PBS or TSLP (two-tailed Mann-Whitney test,
n=12–16 ears). (E) Purified
human neutrophils were pretreated with DMSO or DPI, treated with PBS or TSLP,
and incubated for 2 h with MRSA. CFU was then determined (representative donor
shown in triplicate, statistics shown are using a two-tailed
paired-t test of 3 donors, 3 independent experiments).
*, p < .05; **, p < .01;
***, p < .001; ns = not significant. (b)
Two-tailed Student’s t-test. Data are representative of
3 independent experiments (A, B) or are combined data from two independent
experiments (C&D).