Table 1. Sequences of primer pairs used for amplification of the whole genome of the IBT/VN/2018 strain.

Primer	Sequence (3′–5′)	Position on the genome	%GC	Tm (°C)	Length (bp)
PEDV_1F	ACTTAAARAGATTTTCTATCTACG	1–24	25	47	1,625
PEDV_1R	TTAACGATACTAAGAGTGGC	1606–1625	40	48
PEDV_2F	GCTGGTCATGTTGTTGTTG	1423–1441	49	49	1,783
PEDV_2R	TAGATGTAGTACTTAGGCAC	3187–3206	40	48
PEDV_3F	TTCTCTGATGAAGTCTCTG	2905–3013	42	47	1,871
PEDV_3R	CATGAGCACCTTCCAATCCTG	4755–4776	50	51
PEDV_4F	TTGCATGTGTTGGTGATCGC	4568–4587	50	50	1,580
PEDV_4R	CCGATGCATAATTCATAGTGTC	6127–6148	41	51
PEDV_5F	GATCATGGCACTGGTATGGTGC	5974–5995	52	54	1,675
PEDV_5R	TCTTGGCACCTACACGCATAC	7628–7648	52	54
PEDV_6F	CAGGATTGCAAGAGCACATTG	7456–7476	48	52	1,678
PEDV_6R	AGTACTAGCATACTGACGCAG	9112–9132	48	52
PEDV_7F	CTYATTGCACCATGGTGGG	8908–8926	53	51	1,710
PEDV_7R	AGCTACCACATAAGTGACAG	10598–10617	45	49
PEDV_8F	GCTCTGATTGTTACATCTTGC	10405–10425	43	50.5	1,621
PEDV_8R	CACTTAACTACACGCAGGTC	12007–12026	50	51
PEDV_9F	ATGCTGAGTCCCTGTCATG	11810–11828	53	51	1,617
PEDV_9R	AGTGCTGTCTTATGCTCCGTG	13406–13426	52	54
PEDV_10F	AGGTATAGTTGGTGTTGTCAC	13175–13195	43	50	1,636
PEDV_10R	AACGACGAACTGGTCATCGAC	14790–14810	52	54
PEDV_11F	ACCTCTGGTGATGCAACCAC	14622–14641	55	54	1,670
PEDV_11R	ATACGTGCACCTGGATAGTAC	16271–16291	48	52
PEDV_12F	TACCTCACATAATGTTCAGCC	16061–16081	43	50	1,649
PEDV_12R	TAGTATGTCTGATAGGTTRGCC	17690–17710	41	51
PEDV_13F	TGTTGTCAGACCTGAAGGTTG	17519–17540	48	52	1,615
PEDV_13R	TGTAAGTGACATAAGCACAGC	19113–19133	43	50
PEDV_14F	AGCGTAAGGTAGGACTCAC	18898–18916	53	51	1,617
PEDV_14R	CAACTGTAGCCTTATGCTTAC	20494–20514	43	50.5
PEDV_15F	TGGACAATGTTCTGTACCAG	20301–20322	45	50	1,649
PEDV_15R	AACRTCATCGTCAGTGCCATG	21929–21949	52	54
PEDV_16F	CATACTGCTYTAGGAACAAAYC	21656–21677	40	50	1,664
PEDV_16R	TGTACCACCCTGCCACTTGC	23300–23319	60	55
PEDV_17F	GACCATAGAGTCAGCATTACAAC	23137–23159	43	53.5	1,640
PEDV_17R	TCGTAAGGTTGAAGTCTAGGAC	24756–24777	45	53
PEDV_18F	AAGTGGCCGTGGTGGGTTTG	24617–24636	60	56	1,606
PEDV_18R	AGTGGCCTTGGCGACTGTGAC	26201–26222	62	58
PEDV_19F	TAGCATTCGGTTGTGGCGC	25991–26009	58	53	1,511
PEDV_19R	CACCTGTGAAACAAGAAGCTC	27482–27502	48	52
PEDV_20F	AGTGGAGGAGAATTCCCAAG	27228–27247	50	51	820
PEDV_20R	TGTATCCATATCAACACCGTC	28026–28048	43	51