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. 2021 Aug 30;17(4):812–818. doi: 10.4103/1673-5374.322467

Figure 4.

Figure 4

CircLphn3 protects BBB from hemin insult.

(A, B) Overexpression of circLphn3 attenuated the hemin-induced downregulation of ZO-1, ZO-2, and occludin, but not ZO-3. miR-185-5p transfection aggravated the ZO-1 decreased expression. (C) In the in vitro monolayer transwell BBB model, circLphn3 overexpression attenuated the hemin-induced high lucifer yellow permeability, while miR-185-5p transfection increased the permeability. (D) Immunofluorescent images of ZO-1 (green, Alexa Fluor® 488) in bEnd.3 cells after different interventions. Overexpression of circLphn3 enhanced ZO-1 expression after hemin treatment, whereas miR-185-5p transfection suppressed the protective effect of circLphn3 on ZO-1. Scale bar: 10 µm. All data are presented as mean ± SEM. The experiments were repeated five times. *P < 0.05; #P < 0.05, vs. hemin group (Bonferroni post hoc test). BBB: blood-brain barrier; circLphn3: circular Lphn3; Lphn3: latrophilin 3; ns: not significant; OE: overexpression; Pe: effective permeability; ZO: zona occludens.