Table 1.
Primer sequences
| Gene | Sequence (5’–3’) |
|---|---|
| circLphn3 (divergent) | F: CAC CAA TTC ACC TCG ACT CTG |
| R: AGG GAC ACA TTC ATA CTG CAC | |
| circLphn3 (convergent) | F: TCT CTC CAC CAA TTC ACC TCG |
| R: ACA CTC CTT TTA GCA GTC CAG G | |
| Lphn3 | F: AGC TTT GAA TGC CTG GAG AGA C |
| R: TCG GTC TTC AAA AGG TTA TCA GC | |
| Tjp1 | F: GTT GTC CCT CCT CTG ATA CCT TC |
| R: TGG CAG TGT CAT TCA CAT CTT TC | |
| Tjp2 | F: ATC TAT GCG GTT CCA ATC AAG |
| R: CCT CTG GTG TCC TGG TAA AGT C | |
| Tjp3 | F: GAA ACG AAG AAA CAG CGA AGA G |
| R: GAC AGA TTA GCA CTG GAC ACC C | |
| Ocln | F: GCT GGA TGA CTA CAG AGA GGA GAG |
| R: TAA GGT TTC CGT CTG TCA TAA TCT C | |
| GAPDH | F: GAC ATC AAG AAG GTG GTG AAG C |
| R: GAA GGT GGA AGA GTG GGA GTT |
circLphn3: Circular Lphn3; F: forward; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; Lphn3: latrophilin 3; Ocln: occludin; R: reverse; Tjp: tight junction protein.