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. 2021 Aug 30;17(4):887–897. doi: 10.4103/1673-5374.322475

Figure 6.

Figure 6

FOXO1 enhances the role of MPP+-induced inflammation in microglia (BV2 cells).

(A) A FOXO1 overexpression cell model was constructed in BV2 cells, and the FOXO1 protein level (fold to vector) was measured by western blot. (B) BV2 cells overexpressing FOXO1 were transfected with miR-615-3p and treated with MPP+ (100 μM) for 24 hours. FOXO1 protein expression (fold to MMP+ vector) was measured by western blot. (C) IL-lβ, IL-6, IL-18, and TNF-α levels produced by BV2 cells were measured by ELISA. (D–F) Western blot was used to measure the activation of the pro-oxidant proteins iNOS and COX2 (D), proinflammatory protein phosphorylated NF-κB (E), and NLRP3-ASC-cleaved Caspase-1 inflammasome (F) (fold to control). Data are expressed as mean ± SD. The experiment was repeated three times. †P < 0.05, ††P < 0.01, †††P < 0.001 (Student’s t-test). ASC: Adaptor protein apoptosis-associated speck-like protein containing CARD domain; COX2: cyclooxygenase-2; ELISA: enzyme linked immunosorbent assay; FOXO1: Forkhead box protein O1; IL: interleukin; iNOS: inducible nitric oxide synthase; MPP+: 1-methyl-4-phenylpyridium; NLRP3: NLR family pyrin domain containing 3; TNF-α: tumor necrosis factor-α.