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. 2021 Aug 30;17(4):832–837. doi: 10.4103/1673-5374.322470

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The neuronal degeneration in the region of microinfarcts.

(A, B) The representative photographs from the cerebral cortex showing the Nissl (green, NeuroTrace™ 500/525) and caspase 3 (red, Alexa Fluor 594)-labeling in the cases of control (A) and experimental model (B). (A1, B1) Magnified photographs from the box-indicated regions in panels A and B showing Nissl and caspase 3 labeling in detail, respectively. (A2, B2) Higher magnified photographs from panels A1 and B1. In contrast to the control, neurons are shrunken and caspase 3 labeling is expressed more strongly in the region of microinfarct. The neuronal degeneration in all the model mice presented a similar pattern (n = 6). Green dot in panel B is a lodged fluorescent microsphere on the pial surface. Scale bars: 200 μm in A and B, 50 μm in A1 and B1, 25 μm in A2 and B2.