Figure 1.

OGD/R elevates the expression of MEG3 and ATG7 protein and the autophagy level in HT22 cells.

HT22 cells were induced by OGD for 6 hours, followed by reoxygenation for different durations (12, 24, and 48 hours). The control group was normal HT22 cells without any treatment. (A) Cell viability was measured by the CCK8 assay. (B) The MEG3 expression was quantified by qRT-PCR and normalized to the control group. (C, D) The ATG7 protein level was evaluated by western blotting and normalized to the control group. (E) MDC staining was conducted to determine autophagy (arrows) in HT22 cells (original magnification 200×, scale bar: 100 μm). Autophagy in HT22 cells gradually increased until 48 hours. Data are representative of three independent experiments (mean ± SD). ***P < 0.001, vs. control group (one-way analysis of variance followed by the least significant difference post hoc test). ATG7: Autophagy-related gene 7; CCK8: Cell Counting Kit-8; CI/RI: cerebral ischemia-reperfusion injury; MDC: monodansylcadaverine; MEG3: maternally expressed gene 3; OD: optical density; OGD/R: oxygen and glucose deprivation/reoxygenation; qRT-PCR: quantitative real-time PCR.