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. 2021 Aug 30;17(4):905–910. doi: 10.4103/1673-5374.322477

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Copyright: © Neural Regeneration Research

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Electroacupuncture effects on MOR, β-EP, GAD67, and GAD65 protein expression in the PAG of nicotine-induced hyperalgesic rats.

(A–D) Western blot analysis of MOR (A), β-EP (B), GAD67 (C), and GAD65 (D) in the PAG of rats in the three groups. Data (mean ± SEM) are presented as relative values to control levels (CTR: n = 6, NIC: n = 6, NIC + EA: n = 6). *P < 0.05, **P < 0.01, vs. CTR group; #P < 0.05, ##P < 0.01, vs. NIC group (one-way analysis of variance followed by Newman-Keuls multiple comparison test). CTR: Age-matched control group; NIC: nicotine-exposed group; NIC + EA: nicotine-exposed/electroacupuncture-treated group. GAD65: glutamic acid decarboxylase 65; GAD67: glutamic acid decarboxylase 67; MOR: μ-opioid receptor; PAG: periaqueductal gray; β-EP: β-endorphin.