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. 2021 Aug 30;17(4):898–904. doi: 10.4103/1673-5374.323077

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Copyright: © Neural Regeneration Research

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Effect of P2X4R overexpression and silencing on the number of tyrosine hydroxylase-positive neurons in the substantia nigra pars compacta.

(A) Immunofluorescence staining of TH-positive neurons (red, Alexa Fluor® 555). (B) Number of TH-positive neurons. Data are expressed as mean ± SEM (n = 5 mice/group). **P < 0.01, vs. control group; ††P < 0.01, vs. P2X4R-RNA group; ‡‡P < 0.01, vs. P2X4R-NC + 6-OHDA group; &&P < 0.01, vs. P2X4R-siRNA group; $$P < 0.01, vs. P2X4R-NC + 6-OHDA group (one-way analysis of variance followed by the Student-Newman-Keuls test). Control group: After 1 week of pretreatment with 0.9% physiological saline, ascorbic acid was injected into the SNpc stereotactically; 6-OHDA group: after 1 week of pretreatment with 0.9% physiological saline, 6-OHDA was injected into the SNpc stereotactically to establish PD; P2X4R-NC group: after 1 week of pretreatment with a negative control lentivirus (NC), ascorbic acid was injected into the SNpc stereotactically; P2X4R-NC + 6-OHDA group: after 1 week of pretreatment with the negative control lentivirus, 6-OHDA was injected into the SNpc stereotactically; P2X4R-RNA group: after 1 week of pretreatment with a lentivirus carrying P2X4R, ascorbic acid was injected into the SNpc stereotactically; P2X4R-RNA + 6-OHDA group: after 1 week of pretreatment with the lentivirus carrying P2X4R, 6-OHDA was injected into the SNpc stereotactically; P2X4R-siRNA group: after 1 week of pretreatment with a lentivirus carrying siRNA targeting P2X4R, ascorbic acid was injected into the SNpc stereotactically; P2X4R-siRNA + 6-OHDA group: after 1 week of pretreatment with the lentivirus carrying siRNA targeting P2X4R, 6-OHDA was injected into the SNpc stereotactically. 6-OHDA: 6-Hydroxydopamine; NC: negative control lentivirus; P2X4R: P2X4 receptor; PD: Parkinson’s disease; siRNA: small-interfering RNA; SNpc: substantia nigra pars compacta; TH: tyrosine hydroxylase.