Table 3.
Effect of the lentivirus carrying small interfering RNA for P2X4R on the mRNA levels of P2X4R, NLRP3, Caspase-1, IL-1β, and IL-18 in the substantia nigra pars compacta
| Gene | Control | 6-OHDA | P2X4R-siRNA | P2X4R-siRNA+6-OHDA | P2X4R-NC | P2X4R-NC+6-OHDA |
|---|---|---|---|---|---|---|
| P2X4R | 0.694±0.267 | 1.798±0.056** | 0.494±0.017&& | 1.284±0.089††‡‡ | 0.655±0.030 | 1.848±0.036$$ |
| NLRP3 | 0.730±0.033 | 1.883±0.034** | 0.504±0.013&& | 1.357±0.029††‡‡ | 0.729±0.038 | 1.898±0.050$$ |
| Caspase-1 | 0.727±0.026 | 1.852±0.044** | 0.511±0.016&& | 1.443±0.041††‡‡ | 0.699±0.025 | 1.872±0.026$$ |
| IL-1β | 0.737±0.036 | 1.842±0.046** | 0.505±0.013&& | 1.344±0.034††‡‡ | 0.696±0.022 | 1.857±0.027$$ |
| IL-18 | 0.676±0.026 | 1.845±0.035** | 0.486±0.013&& | 1.349±0.027††‡‡ | 0.640±0.026 | 1.861±0.047$$ |
Control group: After 1 week of pretreatment with 0.9% physiological saline, ascorbic acid was injected into the SNpc stereotactically; 6-OHDA group: after 1 week of pretreatment with 0.9% physiological saline, 6-OHDA was injected into the SNpc stereotactically to establish PD; P2X4R-NC group: after 1 week of pretreatment with a negative control lentivirus (NC), ascorbic acid was injected into the SNpc stereotactically; P2X4R-NC + 6-OHDA group: after 1 week of pretreatment with the negative control lentivirus, 6-OHDA was injected into the SNpc stereotactically; P2X4R-siRNA group: after 1 week of pretreatment with a lentivirus carrying siRNA targeting P2X4R, ascorbic acid was injected into the SNpc stereotactically; P2X4R-siRNA + 6-OHDA group: after 1 week of pretreatment with the lentivirus carrying siRNA targeting P2X4R, 6-OHDA was injected into the SNpc stereotactically. Data are expressed as mean ± SEM (n = 7 mice/group). **P < 0.01, vs. control group; ††P < 0.01, vs. P2X4R-RNA group; ‡‡P < 0.01, vs. P2X4R-NC + 6-OHDA group; &&P < 0.01, vs. P2X4R-NC group; $$P < 0.01, vs. P2X4R-NC group (one-way analysis of variance followed by the Student-Newman-Keuls test). 6-OHDA: 6-Hydroxydopamine; IL: interleukin; NC: negative control lentivirus; NLRP3: Nod-like receptor family protein 3; P2X4R: P2X4 receptor; PD: Parkinson’s disease; SNpc: substantia nigra pars compacta.