FIGURE 2.

(A) De novo pterostilbene production by E. coli TYR-30 harboring the mutant plasmids of pZAC-tal-4cl and pZBK-sts-romt. (B) In vivo and In vitro catalytic efficiency of the evolved pathway enzymes of TAL-4CL and its wild-type enzymes. In vivo activity was defined as the L-tyrosine consuming rate of the whole resting cells and In vitro activity was defined as the L-tyrosine consuming rate of the crude extract. The data represent means of three replicates and error bars represent standard deviations.