FIGURE 3.

(A) De novo resveratrol production by E. coli TYR-30 harboring the mutant plasmids of pZBK-sts and p214C. E. coli TYR-30 harboring pZBK-sts and p214C was set as the control. (B) In vivo and In vitro catalytic efficiency of the evolved STS and its wild-type enzyme. In vivo activity was defined as the resveratrol forming rate of the whole resting cells and In vitro activity was defined as the resveratrol forming rate of the crude extract. The data represent means of three replicates and error bars represent standard deviations.