FIGURE 4.

(A) Production of pterostilbene from resveratrol by E. coli BW25113 harboring the mutant plasmids of pZBK-romt. E. coli BW25113 harboring pZBK-romt was set as the control. (B) In vivo and In vitro catalytic efficiency of the evolved ROMT and its wild-type enzyme. In vivo activity was defined as the pterostilbene forming rate of the whole resting cells and In vitro activity was defined as t the pterostilbene forming rate of the crude extract. The data represent means of three replicates and error bars represent standard deviations.