KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
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| Mouse anti-Tubulin β3 (Tuj1) | Biolegend | Cat# ab801202; RRID: AB_10063408 |
| Rabbit anti-CaMKII (phospho T286) | Abcam | Cat# ab32678; RRID: AB_725893 |
| Rabbit anti-CaMKII | Abcam | Cat# ab52476; RRID:AB_868641 |
| Rabbit anti-CREB (phospho S133) | Abcam | Cat# ab32096; RRID:AB_731734 |
| Rabbit anti-TrkB (phospho T817) | Thermo Fisher Scientific | Cat# MA5-32207; RRID:AB_2809494 |
| Rabbit anti-ZPK/DLK | Thermo Fisher Scientific | Cat# PA5-32173; RRID:AB_2549646 |
| Rabbit anti-c-Jun (phosphor S63) | Cell Signaling Technology | Cat# 2361; RRID:AB_490908 |
| Alexa Fluor 488 AffiniPure Donkey Anti-Mouse IgG (H+L) | Jackson ImmunoResearch Labs | Cat# 715-545-151; RRID:AB_2341099 |
| Alexa Fluor 594 AffiniPure Donkey Anti-Mouse IgG (H+L) | Jackson ImmunoResearch Labs | Cat# 715-585-151; RRID:AB_2340855 |
| Cy3 AffiniPure Donkey Anti-Rabbit IgG (H+L) | Jackson ImmunoResearch Labs | Cat# 711-165-152; RRID:AB_2307443 |
| Alexa Fluor 594 AffiniPure Donkey Anti-Rabbit IgG (H+L) | Jackson ImmunoResearch Labs | Cat# 711-585-152; RRID:AB_2340621 |
| Alexa Fluor 647 AffiniPure Donkey Anti-Rabbit IgG (H+L) | Jackson ImmunoResearch Labs | Cat# 711-605-152; RRID:AB_2492288 |
| CD90.2 (Thy-1.2) Monoclonal Antibody (53-2.1), PE-Cyanine7 | Thermo Fisher Scientific | Cat# 25-0902-82; RRID:AB_469642 |
| Recombinant HRP Anti-GAPDH antibody | Abcam | Cat# ab201822 |
| WesternSure Goat anti-Rabbit HRP | LI-COR Biosciences | Cat# 926-80011; RRID:AB_2721264 |
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| Chemicals, peptides, and recombinant proteins | ||
|
| ||
| CaMKII alpha (phospho T286) peptide | Abcam | Cat# ab115237 |
| CTB, Alexa Fluor 488 Conjugate | Thermo Fisher Scientific | Cat# C22841 |
| Dynabeads® M-450 Epoxy | Thermo Fisher Scientific | Cat# 14011 |
| DAPI | Thermo Fisher Scientific | Cat# 62248 |
| SuperSignal West Atto Ultimate Sensitivity Substrate | Thermo Fisher Scientific | Cat# A38555 |
| N-Methyl-D-aspartic Acid (NMDA) | Millipore Sigma | Cat# 454575 |
| Autocamtide-2 Related Inhibitory Peptide, Myristoylated (AIP) | Millipore Sigma | Cat# 189482 |
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| Experimental models: Cell lines | ||
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| AAVpro 293T Cell Line | Takara Bio | Cat# 632273 |
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| Experimental models: Organisms/strains | ||
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| C57BL/6J | The Jackson Laboratory | Cat# JAX:000664, RRID:IMSR_JAX:000664 |
| GLAST −/− mice | Obtained from Dr. Kohichi Tanaka | N/A |
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| Recombinant DNA | ||
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| ||
| pAAV-GFP | Obtained from Dr. Kevin Park | N/A |
| pAAV-mSncg-GFP | Obtained from Dr. Yang Hu | N/A |
| pcDNA3-A-CREB | Obtained from Dr. Hongbing Wang | N/A |
| pcDNA3-VP16-CREB | Obtained from Dr. Hongbing Wang | N/A |
| pAAV-EBFP | This study | N/A |
| pAAV-mSncg-EBFP | This study | N/A |
| pAAV-mSncg-CaMKIIa T286D | This study | N/A |
| pAAV-CaMKIIα WT | This study | N/A |
| pAAV-CaMKIIα K42R | This study | N/A |
| pAAV-CaMKIIα K42D | This study | N/A |
| pAAV-CaMKIIα T286D | This study | N/A |
| pAAV-CaMKIIα T286A | This study | N/A |
| pAAV-CaMKIIα T286D/T305A/T306A | This study | N/A |
| pAAV-CaMKIIα T286D/T305D/T306D | This study | N/A |
| pAAV-CaMKIIβ WT | This study | N/A |
| pAAV-CaMKIIβ K43R | This study | N/A |
| pAAV-CaMKIIβ K43D | This study | N/A |
| pAAV-CaMKIIβ T287D | This study | N/A |
| pAAV-CaMKIIβ T287A | This study | N/A |
| pAAV-CaMKIIβ T287D/T306A/T307A | This study | N/A |
| pAAV-CaMKIIβ T287D/T306D/T307D | This study | N/A |
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| Software and algorithms | ||
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| GraphPad Prism 9 | GraphPad | https://www.graphpad.com/scientific-software/prism/ |
| ImageJ | Schindelin et al., 2012 | https://imagej.net/software/fiji |
| Blender 2.9 | Blender | https://www.blender.org |