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. Author manuscript; available in PMC: 2021 Oct 21.
Published in final edited form as: Cell Rep. 2021 Oct 12;37(2):109802. doi: 10.1016/j.celrep.2021.109802

Figure 7. Nf1 deletion alters neuronal cell-type proportions in a region-specific manner.

Figure 7.

(A and B) Differences in relative proportion of Ctip2+ (A) and Cux1+ (B) cells across 43 cortical regions and the full isocortex after Nf1 deletion. The top 15 structures sorted by binned p value and fold change are shown (Nf1fl/fl;Emx1-Cre versus Ctrl, mean ± SD, FDR < 0.05). Flattened isocortex displaying percentage change in Nf1fl/fl;Emx1-Cre versus Ctrl are shown on the right. Only significant pixels are colored, and significant regions are bolded and starred (FDR < 0.05).

(C) Association between relative change in Ctip2+ and Cux1+ cell numbers across cortical regions in the Nf1fl/fl;Emx1-Cre.

(D) Representative 2D sections of 1 mm cortical columns from P14 somatosensory cortex after EdU injection at E16.5 showing colocalization with SATB2 (callosal projection neurons) immunolabeling in upper layers. Scale bar, 200 μm.

(E) Quantification of EdU+/SATB2+ cells within 1 mm cortical columns in (D) (n = 3–5 animals, mean ± SEM).