Fig 4. Expression level of klotho does not change during 10 min of hypoxic stimulation in WT and Kl^−/− mice.

Renal hypoxia was induced by clamping the renal artery for 10 min in WT mice and Kl^−/− mice. (A) Western blot analysis demonstrating klotho expression in WT and Kl^−/− mice. Protein levels were normalized to β-actin levels (n = 5 in each group). (B) Representative immunohistochemical staining images showing expression and localization of klotho in WT and Kl^−/− mice. Values are mean ± SD. *P < 0.05, **P < 0.01. Bar = 100 μm.