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. 2000 Feb;20(4):1436–1447. doi: 10.1128/mcb.20.4.1436-1447.2000

FIG. 1.

FIG. 1

Telomerase activity and telomere maintenance in hTERT transductants. (a) TRAP assays of telomerase activity in control (vector) and hTERT-transduced cell lines tested at indicated PD levels. The group of three lanes designated by each number show assay results for 2 μg of extract preincubated with RNase as a control and for 1 and 2 μg of extract assayed. Groups: 1, N/BABE-2F, 50 PD; 2, N/TERT-2F, 48 PD (pre-SGP); 3, N/TERT-1, 57 PD (early SGP); 4, LiF-Ep/BABE-1, 35 PD; 5, LiF-Ep/BABE-2, 31 PD; 6, LiF-Ep/TERT-2, 39 PD (late SGP); 7, LiF-Ep/TERT-1, 45 PD (RDI); 8, OKF6, 18 PD; 9, OKF6/BABE-1, 39 PD; 10, OKF6/TERT-1, 42 PD (beginning of SGP); 11, OKF6/TERT-1, 60 PD (early RDI); 12, OKF6/TERT-1, 107 PD; 13, POE9n/BABE-1, 32 PD; 14, POE9n/BABE-1, 83 PD; 15, POE9n/TERT-1, 38 PD; 16, POE9n/TERT-1, 127 PD. HT, reaction mixture heat treated before thermocycling; IC, internal (positive) control amplification product showing activity of PCR reagents.