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. 2000 Feb;20(4):1436–1447. doi: 10.1128/mcb.20.4.1436-1447.2000

FIG. 3.

FIG. 3

Expression of cell cycle regulatory proteins by hTERT transductants. (a) Western blots comparing parent cell lines with hTERT-immortalized cell lines. “RDI+n” indicates number of population doublings after RDI cells emerged from the SGP population. Lanes: 1, N, 37 PD (mid-life span); 2, N/TERT-1, 135 PD (RDI+68); 3, LiF-Ep, 20 PD (mid-life span); 4, LiF-Ep/TERT-1, 90 PD (RDI+55); 5, OKF6, 19 PD (mid-life span); 6, OKF6/TERT-1, 63 PD (RDI+11); 7, OKF6/TERT-2, 52 PD (RDI+15). The mobility of the protein recognized by each antibody was as expected for the protein, compared with molecular mass markers separated on the same gel (data not shown). (b) Western blot analysis of p16INK4a expression by hTERT-immortalized mesothelial cells and fibroblasts. Life span designations as noted above. Lanes 1, strain N keratinocytes, 33 PD (mid-life span); 2, LP9 mesothelial cells, 41 PD (mid-life span); 3, LP9/TERT-1, 108 PD; 4, S1F/BABE-1, 70 PD (late life span); 5, S1F/TERT-1, 147 PD.