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. Author manuscript; available in PMC: 2021 Oct 21.
Published in final edited form as: Methods Cell Biol. 2019 Dec 10;155:3–31. doi: 10.1016/bs.mcb.2019.10.002
Pros Cons
High yield
Inexpensive procedure
High stability of the mitochondria
Does not require ultracentrifugation		 Low purity
Need for suitable equipment for large volumes of buffers and a cold room
Time consuming procedure