Table 2.
Immunofluorescent staining intensity of CASR, RGS5, and RCAN1 in parathyroid tumors
| Intensity score | High EC50 (n = 18) | Low EC50 (n = 11) | Nonsigmoid (n = 6) | |
|---|---|---|---|---|
| CASR | +++ | 6% (1) | 0% (0) | 33% (2) |
| ++ | 17% (3) | 0% (0) | 0 | |
| + | 44% (8) | 64% (7) | 13% (1) | |
| - | 33% (6) | 36% (4) | 50% (3) | |
| RGS5 | +++ | 17% (3) | 18% (2) | 33% (2) |
| ++ | 0 | 0 | 0 | |
| + | 6% (1) | 18% (2) | 0 | |
| - | 78% (14) | 64% (7) | 67% (4) | |
| RCAN1 | +++ | 0 | (18%) 2 | 67% (4) |
| ++ | 17% (3) | 9% (1) | 0 | |
| + | 39% (7) | 55% (6) | 33% (2) | |
| - | 44% (8) | 18% (2) | 0 |
Threshold-corrected, IgG-background-subtracted pixel counts for each immunofluorescent signal were normalized to cell number to generate a signal intensity score. The mean signal intensity in normal parathyroid tissue is defined as 1+, and a SD value was calculated based on the variation observed among 5 independent normal donor parathyroid glands. Intensity scores more than 1 SD below the normal tissue mean were classified as negative (“-”). Sections within ± 1 SD of mean normal were classified as +1. Sections with intensity scores greater than 2 or 3 SD above the normal tissue mean were classified as 2+ and 3+, respectively.