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Coffee induces AhR-responsive genes in Caco2 cells. Caco2 wild-type and AhR knockout (Caco2-AhRKO) cells were treated with DMSO, 10 nM TCDD, water or chloroform extracts of coffee for 24 h and changes in expression of (A) CYP1A1, CYP1B1, (B) UGT1A1, (C) mRNA were determined by real time PCR. Results are expressed as means ± SE for at least 3 separate determinations per treatment group and significant (p<0.05) induction is indicated (*).
