Skip to main content
. 2021 May 21;29(10):2920–2930. doi: 10.1016/j.ymthe.2021.05.015

Figure 1.

Figure 1

Characterization of EVs

(A) Representative TEM images of EVs isolated from HCT116 cells (Exos) and HCT116 cells treated with OBP-301 (Exo301). Scale bars, 100 nm. (B) HCT116 cells treated with PBS or OBP-301 (MOI of 50) were subjected to immunofluorescence staining for CD9 (Exo marker, red), hexon (adenovirus marker, green), and DAPI (nuclear marker, blue) 24 h after treatment. The right bottom image is a magnified view of the boxed region in the merged image. Scale bars, 20 μm (left upper) and 2 μm (right bottom). (C) Particle size of Exos and Exo301 were measured by DLS. (D) DNAs extracted from EC, Exo301, and EC-Exo301 were subjected to qRT-PCR for the adenovirus E1A gene (n = 3). E1A copy numbers are expressed as fold change relative to Exo301. ∗p < 0.05.