Fig. 5.

The effects of proton pump inhibitors (PPIs) on primary osteoblast matrix mineralisation. Primary osteoblasts were cultured for 28 days in the presence of 0–10 µM lansoprazole, omeprazole, esomeprazole and pantoprazole. A Microscopic images of alizarin red stained mineral associated with nodule formation. Scale bars are 200 μm. B Alizarin red staining. Images are representative of three individual experiments. C Quantification of alizarin red staining, D Alizarin red staining of primary osteoblasts treated with 50 µM pantoprazole. The data are represented as mean ± S.E.M. (n = 3 wells/treatment) P < 0.05*, P < 0.01**, P < 0.001***