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. 2021 May 29;29(10):3072–3092. doi: 10.1016/j.ymthe.2021.05.023

Figure 6.

Figure 6

Neuro-specific hippocampal anti-NKCC1 amiR treatment restores cognitive function in Ts65Dn mice

(A) Left: schematic representation of the experimental timeline: 2–3-month-old WT and Ts65Dn mice were stereotaxically injected in the dorsal hippocampal CA1 region with AAV9 vectors expressing EGFP and control or NKCC1 amiRs from the neuron-specific hSyn promoter. Mice were tested in behavioral experiments between 4 and 6 WPIs. Right: symbol legend for panels (B)–(E). (B) Top, schematic representation of the T-maze test. Bottom, quantification of correct alternations in AAV-injected WT and Ts65Dn mice. (C) Top, schematic representation of the contextual fear-conditioning test. Bottom, quantification of the freezing response in AAV-injected WT and Ts65Dn mice. (D) Top, schematic representation of the novel-object recognition test. Bottom, quantification of the discrimination index in AAV-injected WT and Ts65Dn mice. (E) Top, schematic representation of the object-location test. Bottom, quantification of the discrimination index in AAV-injected WT and Ts65Dn mice. For (A)–(E): symbols indicate values from single animals; lines report group means ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, Tukey post hoc test after two-way ANOVA.